验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, MCF-7 cells |
| Positive IHC detected in | human small intestine tissue, human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SNRPD3 Ready-To-Use IHC Kit. SNRPD3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
10379-1-AP targets SNRPD3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0293 Product name: Recombinant human SNRPD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 14-119 aa of BC000457 Sequence: EGHIVTCETNTGEVYRGKLIEAEDNMNCQMSNITVTYRDGRVAQLEQVYIRGSKIRFLILPDMLKNAPMLKSMKNKNQGSGAGRGKAAILKAQVAARGRGRGMGRG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | small nuclear ribonucleoprotein D3 polypeptide 18kDa |
| 别名 | Sm D3, SMD3, snRNP core protein D3, SNRPD3 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC000457 |
| 基因名称 | SNRPD3 |
| Gene ID (NCBI) | 6634 |
| RRID | AB_2286308 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62318 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Small nuclear ribonucleoprotein D3 polypeptide 18kDa (SNRPD3,synonym: SMD) belongs to the small nuclear ribonucleoprotein core protein family, with molecular weights of the D members: D1,16 kDa; D2, 16.5 kDa and D3 18 kDa. SNRPD3 is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SNRPD3 antibody 10379-1-AP | Download protocol |
| WB protocol for SNRPD3 antibody 10379-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Oncol Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors. |
