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验证数据展示

  • WB analysis of MCF-7 using 10379-1-AP

    WB analysis of MCF-7 using 10379-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10379-1-AP (SNRPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 10379-1-AP

    IHC staining of human small intestine using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SNRPD3 antibody included
  • IHC staining of human small intestine using 10379-1-AP

    IHC staining of human small intestine using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with SNRPD3 antibody included
  • IHC staining of human lung cancer using 10379-1-AP

    IHC staining of human lung cancer using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10379-1-AP (SNRPD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNRPD3 antibody included

SNRPD3 Polyclonal antibody

SNRPD3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10379-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Sm D3, SMD3, snRNP core protein D3, SNRPD3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IHC detected inhuman small intestine tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SNRPD3 Ready-To-Use IHC Kit. SNRPD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10379-1-AP targets SNRPD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 SNRPD3 fusion protein Ag0293 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small nuclear ribonucleoprotein D3 polypeptide 18kDa
别名 Sm D3, SMD3, snRNP core protein D3, SNRPD3
计算分子量 18 kDa
观测分子量 14 kDa
GenBank蛋白编号BC000457
基因名称 SNRPD3
Gene ID (NCBI) 6634
RRIDAB_2286308
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62318
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small nuclear ribonucleoprotein D3 polypeptide 18kDa (SNRPD3,synonym: SMD) belongs to the small nuclear ribonucleoprotein core protein family, with molecular weights of the D members: D1,16 kDa; D2, 16.5 kDa and D3 18 kDa. SNRPD3 is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis.

实验方案

Product Specific Protocols
WB protocol for SNRPD3 antibody 10379-1-APDownload protocol
IHC protocol for SNRPD3 antibody 10379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

CRISPR-based RNA-binding protein mapping in live cells.

Authors - Baiwen Chen
View Article
humanIHC

Transl Oncol

Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors.

Authors - Cunha Isabela Werneck IW
View Article