验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IHC detected in | human cervical cancer tissue, human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16807-1-AP targets SNRPB in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag10298 Product name: Recombinant human SNRPB protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-234 aa of BC003530 Sequence: MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPMPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGKCLQW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | small nuclear ribonucleoprotein polypeptides B and B1 |
| 别名 | Sm protein B/B, Sm protein B/B', Small nuclear ribonucleoprotein associated proteins B and B', Small nuclear ribonucleoprotein-associated proteins B and B', SmB/B |
| 计算分子量 | 234aa,25 kDa; 285aa,30 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC003530 |
| 基因名称 | SNRPB |
| Gene ID (NCBI) | 6628 |
| RRID | AB_2878319 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14678 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SNRPB (Small Nuclear Ribonucleoprotein Polypeptides B and B1) is a core component of the spliceosome, a complex molecular machine that plays a critical role in pre-mRNA splicing. This process is essential for the removal of introns and the joining of exons to produce mature mRNA molecules.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SNRPB antibody 16807-1-AP | Download protocol |
| WB protocol for SNRPB antibody 16807-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation. | ||
Cell Death Dis SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.
| ||
Oncogene The splicing factor SNRPB promotes ovarian cancer progression through regulating aberrant exon skipping of POLA1 and BRCA2
| ||
Aging (Albany NY) SNRPB promotes the progression of hepatocellular carcinoma via regulating cell cycle, oxidative stress, and ferroptosis | ||
Ann Med SNRPB and CEP290, predicting the prognosis of diffuse large B cell lymphoma and associated with tumour immune microenvironment | ||
Adv Sci (Weinh) PRMT5 Maintains Homeostasis of the Intestinal Epithelium by Modulating Cell Proliferation and Survival |
