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验证数据展示

  • WB analysis using 85649-2-RR

    WB analysis using 85649-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85649-2-RR (SNRPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 85649-2-RR

    IF Staining of HepG2 using 85649-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNRPA1 antibody (85649-2-RR, Clone: 243097D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 85649-2-RR

    IF Staining of HeLa using 85649-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNRPA1 antibody (85649-2-RR, Clone: 243097D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SNRPA1 Recombinant antibody

SNRPA1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85649-2-RR

产品说明书

CloneNo. 243097D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

U2 small nuclear ribonucleoprotein A', U2 snRNP A, U2 snRNP A'

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, Raji cells, HeLa cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy SNRPA1 Ready-To-Use IHC Kit. SNRPA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85649-2-RR targets SNRPA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SNRPA1 fusion protein Ag10790 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 small nuclear ribonucleoprotein polypeptide A'
别名 U2 small nuclear ribonucleoprotein A', U2 snRNP A, U2 snRNP A'
计算分子量 255 aa, 28 kDa
观测分子量28 kDa
GenBank蛋白编号BC022816
基因名称 SNRPA1
Gene ID (NCBI) 6627
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09661
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small nuclear ribonucleoprotein polypeptide a-prime(SNRPA1) is part of the U2 snRNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. It may be required for cell division, and helps the A' protein to bind stem loop IV of U2 snRNA. This is a rabbit polyclonal antibody raised against the full length SNRPA1 of human origin.

实验方案

Product Specific Protocols
WB protocol for SNRPA1 antibody 85649-2-RRDownload protocol
IF protocol for SNRPA1 antibody 85649-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols