验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, Raji cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung tissue, human brain tissue, human kidney tissue, human liver tissue, human ovary tissue, human placenta tissue, human spleen tissue, human stomach cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SNRPA1 Ready-To-Use IHC Kit. SNRPA1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
17368-1-AP targets SNRPA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | SNRPA1 fusion protein Ag10790 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | small nuclear ribonucleoprotein polypeptide A' |
| 别名 | U2 snRNP A', U2 snRNP A, U2 small nuclear ribonucleoprotein A' |
| 计算分子量 | 255 aa, 28 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC022816 |
| 基因名称 | SNRPA1 |
| Gene ID (NCBI) | 6627 |
| RRID | AB_2193724 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09661 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Small nuclear ribonucleoprotein polypeptide a-prime(SNRPA1) is part of the U2 snRNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. It may be required for cell division, and helps the A' protein to bind stem loop IV of U2 snRNA. This is a rabbit polyclonal antibody raised against the full length SNRPA1 of human origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNRPA1 antibody 17368-1-AP | Download protocol |
| IHC protocol for SNRPA1 antibody 17368-1-AP | Download protocol |
| IF protocol for SNRPA1 antibody 17368-1-AP | Download protocol |
| IP protocol for SNRPA1 antibody 17368-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Inactivation of the tumor suppressor p53 by long noncoding RNA RMRP. | ||
Cell Chem Biol Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer. | ||
STAR Protoc Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis. | ||
Clin Respir J High SNRPA1 expression leads to poor prognosis in patients with lung adenocarcinoma |
