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验证数据展示

  • WB analysis using 86259-3-RR (same clone as 86259-3-PBS)

    WB analysis using 86259-3-RR (same clone as 86259-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86259-3-RR (SNRPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86259-3-PBS in a different storage buffer formulation.

  • WB analysis using 86259-3-RR (same clone as 86259-3-PBS)

    WB analysis using 86259-3-RR (same clone as 86259-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86259-3-RR (SNRPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86259-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86259-3-RR (same clone as 86259-3-PBS)

    IF Staining of HepG2 using 86259-3-RR (same clone as 86259-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNRPA antibody (86259-3-RR, Clone: 251055G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86259-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86259-3-RR

    Affinity and Kinetic Characterization of 86259-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86259-3-RR against Human SNRPA were performed. The affinity constant is below 1 pM.

SNRPA Recombinant monoclonal antibody, PBS Only

SNRPA Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86259-3-PBS

产品说明书

CloneNo. 251055G1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

U1 A, U1 snRNP A, U1A, U1-A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86259-3-PBS targets SNRPA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0283

Product name: Recombinant human SNRPA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-258 aa of BC000405

Sequence: KKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 small nuclear ribonucleoprotein polypeptide A
别名 U1 A, U1 snRNP A, U1A, U1-A
计算分子量 31 kDa
观测分子量31 kDa
GenBank蛋白编号BC000405
基因名称 SNRPA
Gene ID (NCBI) 6626
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09012
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP A1 functions in the packaging of nascent RNA polymerase II transcripts and participates in a variety of nuclear and cytoplasmic processes that modulate gene expression. The RNA binding characteristics of hnRNP A1 suggest that it can modulate the expression of specific genes.

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