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验证数据展示

  • IHC staining of mouse cerebellum using 19890-1-AP

    IHC staining of mouse cerebellum using 19890-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19890-1-AP (SNRNP25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 19890-1-AP

    IHC staining of mouse cerebellum using 19890-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19890-1-AP (SNRNP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNRNP25 Polyclonal antibody

SNRNP25 Polyclonal Antibody for IHC, ELISA
Cat No. 19890-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

C16orf33, Minus 99 protein, SNRNP25, U11/U12 25K, U11/U12 snRNP 25 kDa protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19890-1-AP targets SNRNP25 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13724

Product name: Recombinant human SNRNP25 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-132 aa of BC001381

Sequence: MDVFQEGLAMVVQDPLLCDLPIQVTLEEVNSQIALEYGQAMTVRVCKMDGEVMPVVVVQSATVLDLKKAIQRYVQLKQEREGGIQHISWSYVWRTYHLTSAGEKLTEDRKKLRDYGIRNRDEVSFIKKLRQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small nuclear ribonucleoprotein 25kDa (U11/U12)
别名 C16orf33, Minus 99 protein, SNRNP25, U11/U12 25K, U11/U12 snRNP 25 kDa protein
计算分子量 132 aa, 15 kDa
GenBank蛋白编号BC001381
基因名称 SNRNP25
Gene ID (NCBI) 79622
RRIDAB_3085590
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNRNP25, small nuclear ribonucleoprotein U11/U12 subunit 25, encodes a 25K protein that is a component of the U12-type spliceosome. Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. LRP1-SNRNP25 fusion gene promotes osteosarcoma cell invasion and migration (PMID: 26738504, PMID: 25300797).

实验方案

Product Specific Protocols
IHC protocol for SNRNP25 antibody 19890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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