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验证数据展示

  • IHC staining of mouse brain using 13914-1-AP

    IHC staining of mouse brain using 13914-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13914-1-AP (SNN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 13914-1-AP

    IF Staining of U2OS using 13914-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 13914-1-AP (SNN antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SNN Polyclonal antibody

SNN Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 13914-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

Stannin, Hemotin, AG8_1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

The antibody is only suitable for IF and IHC detection.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13914-1-AP targets SNN in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4909

Product name: Recombinant human SNN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-88 aa of BC036443

Sequence: MSIMDHSPTTGVVTVIVILIAIAALGALILGCWCYLRLQRISQSEDEESIVGDGETKEPFLLVQYSAKGPCVERKAKLMTPNGPEVHG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stannin
别名 Stannin, Hemotin, AG8_1
计算分子量 9 kDa
GenBank蛋白编号BC036443
基因名称 SNN
Gene ID (NCBI) 8303
RRIDAB_2918033
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75324
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stannin (Snn) is discovered using subtractive hybridization methodology designed to find gene products related to selective organotin toxicity and apoptosis. It is present in TMT-sensitive cells and may play a role in the selective toxicity of organotin compounds.

实验方案

Product Specific Protocols
IF protocol for SNN antibody 13914-1-APDownload protocol
IHC protocol for SNN antibody 13914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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