验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29890-1-AP targets SNIP/p140Cap in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag31857 Product name: Recombinant human SNIP protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: RSFPSSHGLTTTRTGEVVVTSKKDSAFIKKAESEELEVQKPQVKLRRAVSEVARPASTPPIMASAIKDEDDEDRIIAELESGGGSVPPMKVVTPGASRLKAAQGQAGSPDKSKHGKQRAEYMRIQAQQQATKPSKEMSGSNETSSPVSEKPSASRTSIPVLTSFGARNSSIS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SNAP25-interacting protein |
| 别名 | KIAA1684, p130Cas associated protein, P140, p140Cap, SNAP 25 interacting protein, SNAP25 interacting protein, SNIP, SRCIN1 |
| 计算分子量 | 127 kDa |
| 观测分子量 | 145 kDa |
| 基因名称 | SNIP |
| Gene ID (NCBI) | 80725 |
| RRID | AB_3086180 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9C0H9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SNIP, also named SRC kinase signaling inhibitor 1 (SRCIN1) and p140 Cas-associated protein (p140CAP), contains two regions of highly charged amino acids, two proline-rich regions, and two coiled-coil domains. SNIP plays a role in SRC inactivation and acts as a tumor suppressor gene in cancers. MiR-32 suppresses the expression of SNIP, thereby suppressing proliferation and epithelial-mesenchymal transition (EMT) of human liver cancer cells (PMID: 28550679).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNIP/p140Cap antibody 29890-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
