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SNF2L Polyclonal antibody

SNF2L Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29461-1-AP

Print datasheet

Synonyms

ATP dependent helicase SMARCA1, DKFZp686D1623, FLJ41547, ISWI, NURF140, SMARCA1, SNF2L, SNF2L1, SNF2LB, SWI, SWI2

验证数据展示

  • WB analysis using 29461-1-AP

    WB analysis using 29461-1-AP

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 29461-1-AP

    IHC staining of mouse lung using 29461-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCA1/SNF2L antibody included
  • IHC staining of rat lung using 29461-1-AP

    IHC staining of rat lung using 29461-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCA1/SNF2L antibody included

经过测试的应用

Positive WB detected inU-87 MG cells, U-251 cells, SH-SY5Y cells
Positive IHC detected inmouse lung tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SMARCA1/SNF2L Ready-To-Use IHC Kit. SMARCA1/SNF2L primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29461-1-AP targets SNF2L in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen SNF2L fusion protein Ag30657 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
Synonyms ATP dependent helicase SMARCA1, DKFZp686D1623, FLJ41547, ISWI, NURF140, SMARCA1, SNF2L, SNF2L1, SNF2LB, SWI, SWI2
Calculated Molecular Weight 123 kDa
Observed Molecular Weight120-130 KDa
GenBank Accession NumberNM_003069
Gene Symbol SMARCA1
Gene ID (NCBI) 6594
RRIDAB_2918310
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28370
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNF2L, also named as SMARCA1, NURF140 and ISWI, encodes the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription (PMID: 28801535). SNF2L was shown to promote neurite outgrowth as well as regulate the human engrailed homeotic genes, important regulators of brain development (PMID: 15310751). The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells (PMID: 25462860). SNF2L has 2 isoforms with MW of 123 kDa and 121 kDa, and can be detected as 120-130 kDa.

实验方案

Product Specific Protocols
WB protocol for SNF2L antibody 29461-1-APDownload protocol
IHC protocol for SNF2L antibody 29461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

BAP18 facilitates CTCF-mediated chromatin accessible to regulate enhancer activity in breast cancer

Authors - Ge Sun
View Article