验证数据展示

WB analysis using 10842-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10842-1-AP (alpha-synuclein antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 10842-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10842-1-AP (Alpha Synuclein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 10842-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10842-1-AP (Alpha Synuclein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Alpha Synuclein antibody included
IHC staining of mouse brain using 10842-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10842-1-AP (Alpha Synuclein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Alpha Synuclein antibody included
IF Staining of SH-SY5Y using 10842-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Alpha Synuclein antibody (10842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of SH-SY5Y using 10842-1-AP
Immunofluorescent analysis of SH-SY5Y cells using 10842-1-AP (alpha-synuclein antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 10842-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Alpha Synuclein antibody (10842-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat brain using 10842-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using Alpha Synuclein antibody (10842-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of SH-SY5Y using 10842-1-AP
1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Alpha Synuclein Polyclonal antibody (10842-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Featured Product
KD/KO Validated

Alpha Synuclein Polyclonal antibody

Alpha Synuclein Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Cat No. 10842-1-AP

产品说明书

引用文献(128)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA and More (3)

别名

1, a-synuclein, SNCA, alpha syn, alpha-syn

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 750

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (128)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected in	mouse brain tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive IF-Fro detected in	rat brain tissue
Positive IF/ICC detected in	SH-SY5Y cells
Positive FC (Intra) detected in	SH-SY5Y cells

Planning an IHC experiment? We recommend our IHCeasy Alpha Synuclein Ready-To-Use IHC Kit. Alpha Synuclein primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 92 publications below
IHC	See 33 publications below
IF	See 30 publications below
IP	See 1 publications below
ELISA	See 2 publications below
CoIP	See 1 publications below

产品信息

10842-1-AP targets Alpha Synuclein in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, ELISA
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, monkey, yeast, zebra finch
免疫原	CatNo: Ag1285 Product name: Recombinant human a-Synuclein protein Source: e coli.-derived, PKG Tag: GST Domain: 1-140 aa of BC013293 Sequence: MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	synuclein, alpha (non A4 component of amyloid precursor)
别名	1, a-synuclein, SNCA, alpha syn, alpha-syn
计算分子量	14 kDa
观测分子量	15-19 kDa
GenBank蛋白编号	BC013293
基因名称	Alpha Synuclein
Gene ID (NCBI)	6622
RRID	AB_2192672
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P37840
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. Α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.

实验方案

Product Specific Protocols
FC protocol for Alpha Synuclein antibody 10842-1-AP	Download protocol
IF protocol for Alpha Synuclein antibody 10842-1-AP	Download protocol
IHC protocol for Alpha Synuclein antibody 10842-1-AP	Download protocol
WB protocol for Alpha Synuclein antibody 10842-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IF	Mol Neurodegener Posttranslational modification and mutation of histidine 50 trigger alpha synuclein aggregation and toxicity. Authors - Wei Xiang View Article
human	WB	Mol Neurodegener Recombinant cathepsins B and L promote α-synuclein clearance and restore lysosomal function in human and murine models with α-synuclein pathology Authors - Denise Balta View Article
human	WB	Transl Neurodegener Reciprocal effects of alpha-synuclein aggregation and lysosomal homeostasis in synucleinopathy models Authors - Alice Drobny View Article
human	WB	Autophagy R406 and its structural analogs reduce SNCA/α-synuclein levels via autophagic degradation Authors - Chao Zhong View Article
mouse	WB	J Nanobiotechnology Umbilical cord blood-derived exosomes attenuate dopaminergic neuron damage of Parkinson's disease mouse model Authors - Junjie Ye View Article
mouse	WB	Theranostics Chronic intermittent hypoxia increases Parkinson's disease susceptibility via PPARα-mediated lipid droplet-mitochondrial dysfunction. Authors - Ming-Rui Zhai View Article

验证数据展示

WB analysis using 10842-1-AP

WB analysis using 10842-1-AP

IHC staining of mouse brain using 10842-1-AP

IHC staining of mouse brain using 10842-1-AP

IF Staining of SH-SY5Y using 10842-1-AP

IF Staining of SH-SY5Y using 10842-1-AP

IF Staining of mouse brain using 10842-1-AP

IF Staining of rat brain using 10842-1-AP

FC experiment of SH-SY5Y using 10842-1-AP

Alpha Synuclein Polyclonal antibody

Alpha Synuclein Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Posttranslational modification and mutation of histidine 50 trigger alpha synuclein aggregation and toxicity.

Recombinant cathepsins B and L promote α-synuclein clearance and restore lysosomal function in human and murine models with α-synuclein pathology

Reciprocal effects of alpha-synuclein aggregation and lysosomal homeostasis in synucleinopathy models

R406 and its structural analogs reduce SNCA/α-synuclein levels via autophagic degradation

Umbilical cord blood-derived exosomes attenuate dopaminergic neuron damage of Parkinson's disease mouse model

Chronic intermittent hypoxia increases Parkinson's disease susceptibility via PPARα-mediated lipid droplet-mitochondrial dysfunction.

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