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验证数据展示

  • WB analysis using 25928-1-AP

    WB analysis using 25928-1-AP

    37°C incubated and boiled mouse brain tissues were subjected to SDS PAGE followed by western blot with 25928-1-AP (SNAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 25928-1-AP

    IHC staining of mouse pancreas using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC38A2 antibody included
  • IHC staining of mouse pancreas using 25928-1-AP

    IHC staining of mouse pancreas using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC38A2 antibody included

SNAT2 Polyclonal antibody

SNAT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25928-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SLC38A2, SAT2, Protein 40-9-1, Protein 40 9 1, PRO1068

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in37°C incubated mouse brain tissue
Positive IHC detected inmouse pancreas tissue, human cervical cancer tissue, human kidney tissue, rat pancreas tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SLC38A2 Ready-To-Use IHC Kit. SLC38A2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25928-1-AP targets SNAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag23082

Product name: Recombinant human SLC38A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-50 aa of BC040342

Sequence: MKKAEMGRFSISPDEDSSSYSSNSDFNYSYPTKQAALKSHYADVDPENQN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 38, member 2
别名 SLC38A2, SAT2, Protein 40-9-1, Protein 40 9 1, PRO1068
观测分子量45 kDa, 56 kDa
GenBank蛋白编号BC040342
基因名称 SLC38A2
Gene ID (NCBI) 54407
RRIDAB_3085830
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QD8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNAT2 (Sodium-dependent neutral amino acid transporter-2), the ubiquitous member of SLC38 family, accounts for the activity of transport system A for neutral amino acids in most mammalian tissues (PMID: 16734764). SNAT2 mediates uptake of neutral α-amino acids and are expressed in central neurons (PMID: 19240036). SNAT2 is expressed in breast cancer cell lines. SLC38A2 also acts as a selective target for inhibiting growth of Gln-dependent breast cancer cell lines (PMID: 33028955).

实验方案

Product Specific Protocols
WB protocol for SNAT2 antibody 25928-1-APDownload protocol
IHC protocol for SNAT2 antibody 25928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cancer Lett

CircMFN2/miR-361-3p/ELK1 feedback loop promotes glutaminolysis and the progression of hepatocellular carcinoma

Authors - Xiaopei Hao
View Article