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SNAT2 Polyclonal antibody

SNAT2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25928-1-AP

Print datasheet

Synonyms

Amino acid transporter A2, ATA2, KIAA1382, PRO1068, Protein 40 9 1, SAT2, SLC38A2, SNAT2, System A transporter 1

验证数据展示

  • WB analysis using 25928-1-AP

    WB analysis using 25928-1-AP

    37°C incubated and boiled mouse brain tissues were subjected to SDS PAGE followed by western blot with 25928-1-AP (SNAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 25928-1-AP

    IHC staining of mouse pancreas using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC38A2 antibody included
  • IHC staining of mouse pancreas using 25928-1-AP

    IHC staining of mouse pancreas using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC38A2 antibody included

经过测试的应用

Positive WB detected in37°C incubated mouse brain tissue
Positive IHC detected inmouse pancreas tissue, human cervical cancer tissue, human kidney tissue, rat pancreas tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SLC38A2 Ready-To-Use IHC Kit. SLC38A2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25928-1-AP targets SNAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SNAT2 fusion protein Ag23082 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 38, member 2
Synonyms Amino acid transporter A2, ATA2, KIAA1382, PRO1068, Protein 40 9 1, SAT2, SLC38A2, SNAT2, System A transporter 1
Observed Molecular Weight45 kDa, 56 kDa
GenBank Accession NumberBC040342
Gene Symbol SLC38A2
Gene ID (NCBI) 54407
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QD8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNAT2 (Sodium-dependent neutral amino acid transporter-2), the ubiquitous member of SLC38 family, accounts for the activity of transport system A for neutral amino acids in most mammalian tissues (PMID: 16734764). SNAT2 mediates uptake of neutral α-amino acids and are expressed in central neurons (PMID: 19240036). SNAT2 is expressed in breast cancer cell lines. SLC38A2 also acts as a selective target for inhibiting growth of Gln-dependent breast cancer cell lines (PMID: 33028955).

实验方案

Product Specific Protocols
WB protocol for SNAT2 antibody 25928-1-APDownload protocol
IHC protocol for SNAT2 antibody 25928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols