验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
14093-1-AP targets SNAP91 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, pig |
| 免疫原 |
CatNo: Ag5224 Product name: Recombinant human SNAP91 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 251-600 aa of BC060818 Sequence: LKVAEAPSSLMETLEQHLNTLEGKKPGNKSGAPSPLSKSSPATTVTSPNSTPAKTIDTSPPVDLFATASAAVPVSTSKPSSDLLDLQPDFSSGGAAAAAAPAPPPPAGGATAWGGFGGSFMAPSPSPVTPAQNNLLQPNFEAAFGTTPSTSSSSSFDPSVFDGLGDLLMPTMAPAGQPAPVSMVPPSPAMAASKALGSDLDSSLASLVGNLGISGTTTKKGDLQWNAGEKKLTGGANWQPKVAPATWSAGVPPSAPLQGAVPPTSSVPPVAGAPSVGQPGAGFGMPPAGTGMPMMPQQPVMFAQPMMRPPFGAAAVPGTQLSPSPTPASQSPKKPPAKDPLADLNIKDFL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | synaptosomal-associated protein, 91kDa homolog (mouse) |
| 别名 | Phosphoprotein F1-20, KIAA0656, Clathrin coat-associated protein AP180, Clathrin coat assembly protein AP180, 91 kDa synaptosomal-associated protein |
| 计算分子量 | 92.5 kDa |
| 观测分子量 | 160 kDa |
| GenBank蛋白编号 | BC060818 |
| 基因名称 | SNAP91 |
| Gene ID (NCBI) | 9892 |
| RRID | AB_2923560 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60641 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SNAP91, also known as AP180 or CALM, is a clathrin-coat assembly protein that sequesters clathrin. SNAP91/AP180 is required for clathrin-dependent endocytosis and SV reformation (PMID: 26903854; PMID: 31843760).The AP180 N-terminal homology (ANTH) domains is crucially involved in membrane budding processes, along with and Epsin N-terminal homology (ENTH) domains (PMID: 29774507).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SNAP91 antibody 14093-1-AP | Download protocol |
| IHC protocol for SNAP91 antibody 14093-1-AP | Download protocol |
| WB protocol for SNAP91 antibody 14093-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Emerg Microbes Infect CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis | ||
Mol Med Rep miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase. |
