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SNAP25 Monoclonal antibody, PBS Only

SNAP25 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3E4B7

Cat No : 60159-1-PBS

Print datasheet

Synonyms

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

验证数据展示

  • WB analysis of pig brain using 60159-1-Ig (same clone as 60159-1-PBS)

    WB analysis of pig brain using 60159-1-Ig (same clone as 60159-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 60159-1-Ig (same clone as 60159-1-PBS)

    IP experiment of mouse brain using 60159-1-Ig (same clone as 60159-1-PBS)

    IP result of anti-SNAP25 (IP:60159-1-Ig, 3ug; Detection:60159-1-Ig 1:500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 60159-1-Ig (same clone as 60159-1-PBS)

    IHC staining of rat brain using 60159-1-Ig (same clone as 60159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 60159-1-Ig (same clone as 60159-1-PBS)

    IHC staining of rat brain using 60159-1-Ig (same clone as 60159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-12 using 60159-1-Ig (same clone as 60159-1-PBS)

    IF Staining of PC-12 using 60159-1-Ig (same clone as 60159-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (60159-1-Ig, Clone: 3E4B7 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), SNAP25 antibody (14903-1-AP, green). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

产品信息

60159-1-PBS targets SNAP25 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF
Tested Reactivity human, mouse, rat, pig
Immunogen SNAP25 fusion protein Ag6695 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name synaptosomal-associated protein, 25kDa
Synonyms RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC010647
Gene Symbol SNAP25
Gene ID (NCBI) 6616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60880
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.