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验证数据展示

  • WB analysis using 81584-5-RR (same clone as 81584-5-PBS)

    WB analysis using 81584-5-RR (same clone as 81584-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81584-5-RR (SNAI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81584-5-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 81584-5-RR (same clone as 81584-5-PBS)

    FC experiment of A549 using 81584-5-RR (same clone as 81584-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SNAI1 Recombinant antibody (81584-5-RR, Clone:240164C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 81584-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81584-5-RR

    Affinity and Kinetic Characterization of 81584-5-RR

    Biolayer interferometry (BLl) kinetic assays of 81584-5-RR against Human SNAI1 were performed. The affinity constant is 0.64 nM.

SNAI1 Recombinant monoclonal antibody, PBS Only

SNAI1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81584-5-PBS

产品说明书

CloneNo. 240164C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

240164C11, SLUGH 2, SLUGH2, SNAI 1, SNAIL1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

81584-5-PBS targets SNAI1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24248

Product name: Recombinant human SNAI1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 22-164 aa of BC012910

Sequence: LQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLIWDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSSLEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 snail homolog 1 (Drosophila)
别名 240164C11, SLUGH 2, SLUGH2, SNAI 1, SNAIL1
计算分子量 264 aa, 29 kDa
GenBank蛋白编号BC012910
基因名称 SNAI1
Gene ID (NCBI) 6615
RRIDAB_3670500
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO95863
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

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