SMYD3 Monoclonal antibody
SMYD3 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B5C10
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, COLO 320 cells, HEK-293 cells, MCF-7 cells |
| Positive IHC detected in | human pancreas cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66330-1-Ig targets SMYD3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Tested Reactivity | human |
| Immunogen | SMYD3 fusion protein Ag2624 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | SET and MYND domain containing 3 |
| Synonyms | SMYD3, ZMYND1, ZNFN3A1 |
| Calculated Molecular Weight | 428aa,49 kDa; 369aa,42 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC031010 |
| Gene Symbol | SMYD3 |
| Gene ID (NCBI) | 64754 |
| RRID | AB_2881711 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9H7B4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer(PMID:16155568). Mainly cytoplasmic when cells are arrested at G0/G1, accumulates in the nucleus at S phase and G2/M(Uniprot).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMYD3 antibody 66330-1-Ig | Download protocol |
| IHC protocol for SMYD3 antibody 66330-1-Ig | Download protocol |
| IF protocol for SMYD3 antibody 66330-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
