验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, COLO 320 cells, HeLa cells, HepG2 cells |
Positive IP detected in | COLO 320 cells |
Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12011-1-AP targets SMYD3 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, ChIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | SMYD3 fusion protein Ag2624 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | SET and MYND domain containing 3 |
别名 | ZMYND1, Zinc finger MYND domain-containing protein 1, SET and MYND domain-containing protein 3, Histone-lysine N-methyltransferase SMYD3, EC:2.1.1.354 |
计算分子量 | 428aa,49 kDa; 369aa,42 kDa |
观测分子量 | 42-45 kDa |
GenBank蛋白编号 | BC031010 |
基因名称 | SMYD3 |
Gene ID (NCBI) | 64754 |
RRID | AB_2193981 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9H7B4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) . SMYD3 exists some isoforms with MV 49, 43 and 30 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for SMYD3 antibody 12011-1-AP | Download protocol |
IHC protocol for SMYD3 antibody 12011-1-AP | Download protocol |
IF protocol for SMYD3 antibody 12011-1-AP | Download protocol |
IP protocol for SMYD3 antibody 12011-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Acta Pharm Sin B SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.
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Carcinogenesis miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. | ||
Int J Androl Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines. | ||
Cell Physiol Biochem iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins. | ||
Oncogenesis SMYD3 promotes endometrial cancer through epigenetic regulation of LIG4/XRCC4/XLF complex in non-homologous end joining repair |