验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cell, HEK-293 cells, C2C12 cells, U2OS cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | rat heart tissue, human kidney tissue, human lung cancer tissue, human ovary tumor tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21290-1-AP targets SMYD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag15823 Product name: Recombinant human SMYD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC098305 Sequence: MRAEGLGGLERFCSPGKGRGLRALQPFQVGDLLFSCPAYAYVLTVNERGNHCEYCFTRKEGLSKCGRCKQAFYCNVECQKEDWPMHKLECSPMVVFGENWNPSETVRLTARILAKQKIHPERTPSEKLLAVKEFESHLDKLDNEKKDLIQSDIAALHHFYSKHLGFPDNDSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIKPGEEVFTSYIDLLYPTEDRNDRLRDSYFFTCECQECTTKDK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SET and MYND domain containing 2 |
| 别名 | EC:2.1.1.-, EC:2.1.1.354, Histone methyltransferase SMYD2, HSKM B, HSKM-B |
| 计算分子量 | 433 aa, 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC098305 |
| 基因名称 | SMYD2 |
| Gene ID (NCBI) | 56950 |
| RRID | AB_10733881 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NRG4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMYD2, also known as SET and MYND domain containing 2, encodes an enzyme called N-lysine methyltransferase SMYD2, which plays a crucial role in cellular processes, including histone methylation, non-histone protein methylation, cell proliferation and differentiation, DNA repair (PMID: 18065756, PMID: 36191822). Aberrant SMYD2 expression has been linked to various cancers, including colon cancer, lung cancer, and cervical cancer. Smyd2 is strongly expressed in muscle cells with highest expression in the neonatal heart (PMID: 25125178).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SMYD2 antibody 21290-1-AP | Download protocol |
| IHC protocol for SMYD2 antibody 21290-1-AP | Download protocol |
| WB protocol for SMYD2 antibody 21290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Inhibition of SMYD2 suppresses tumor progression by down-regulating microRNA-125b and attenuates multi-drug resistance in renal cell carcinoma.
| ||
Acta Pharm Sin B The lysine methyltransferase SMYD2 facilitates neointimal hyperplasia by regulating the HDAC3-SRF axis | ||
Cell Death Dis Histone methyltransferase Smyd2 drives adipogenesis via regulating STAT3 phosphorylation
| ||
Cell Death Dis SMYD2 targets RIPK1 and restricts TNF-induced apoptosis and necroptosis to support colon tumor growth. | ||
Aging (Albany NY) Histone methyltransferase Smyd2 drives vascular aging by its enhancer-dependent activity
| ||
J Cachexia Sarcopenia Muscle MyoMed205 Counteracts Titin Hyperphosphorylation and the Expression of Contraction-Regulating Proteins in a Rat Model of HFpEF |
