验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, A549 cells, NIH/3T3 cells, PC-12 cells, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
87769-1-RR targets SMURF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | SMAD specific E3 ubiquitin protein ligase 1 |
| 别名 | E3 ubiquitin-protein ligase SMURF1, EC:2.3.2.26, HECT-type E3 ubiquitin transferase SMURF1, hSMURF1, KIAA1625 |
| 计算分子量 | 86 kDa |
| 观测分子量 | 90-100 kDa |
| GenBank蛋白编号 | NM_020429 |
| 基因名称 | SMURF1 |
| Gene ID (NCBI) | 57154 |
| RRID | AB_3745744 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9HCE7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. The antibody is specific to SMURF1. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMURF1 antibody 87769-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
