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SMUG1 Polyclonal antibody

SMUG1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 19857-1-AP

Print datasheet

Synonyms

FDG, HMUDG, SMUG1, UNG3

验证数据展示

  • WB analysis using 19857-1-AP

    WB analysis using 19857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19857-1-AP (SMUG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCOLO 320 cells, HEK-293 cells, HeLa cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19857-1-AP targets SMUG1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Tested Reactivity human
Immunogen SMUG1 fusion protein Ag13934 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name single-strand-selective monofunctional uracil-DNA glycosylase 1
Synonyms FDG, HMUDG, SMUG1, UNG3
Calculated Molecular Weight 177 aa, 20 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC000417
Gene Symbol SMUG1
Gene ID (NCBI) 23583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53HV7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5). This antibody is specific to UNG.

实验方案

Product Specific Protocols
WB protocol for SMUG1 antibody 19857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols