验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HEK-293 cells, HeLa cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19857-1-AP targets SMUG1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | SMUG1 fusion protein Ag13934 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | single-strand-selective monofunctional uracil-DNA glycosylase 1 |
| 别名 | FDG, HMUDG, SMUG1, UNG3 |
| 计算分子量 | 177 aa, 20 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC000417 |
| 基因名称 | SMUG1 |
| Gene ID (NCBI) | 23583 |
| RRID | AB_3085589 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53HV7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5). This antibody is specific to UNG.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMUG1 antibody 19857-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |