验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, human heart tissue, Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
| IHC | See 2 publications below |
产品信息
15979-1-AP targets Spermine synthase in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8767 Product name: Recombinant human SMS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC009898 Sequence: MAAARHSTLDFMLGAKADGETILKGLQSIFQEQGMAESVHTWQDHGYLATYTNKNGSFANLRIYPHGLVLLDLQSYDGDAQGKEEIDSILNKVEERMKELSQDSTGRVKRLPPIVRGGAIDRYWPTADGRLVEYDIDEVVYDEDSPYQNIKILHSKQFGNILILSGDVNLAESDLAYTRAIMGSGKEDYTGKDVLILGGGDGGILCEIVKLKPKMVTMVEIDQMVIDGCKKYMRKTCGDVLDNLKGDCYQVLIEDCIPVLKRYAKEGREFDYVINDLTAVPISTSPEEDSTWEFLRLILDLSMKVLKQDGKYFTQGNCVNLTEALSLYEEQLGRLYCPVEFSKEIVCVPSYLELWVFYTVWKKAKP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spermine synthase |
| 别名 | SMS, EC:2.5.1.22, MRSR, SPMSY, SpS |
| 计算分子量 | 366 aa, 41 kDa |
| 观测分子量 | 41 kDa, 60 kDa |
| GenBank蛋白编号 | BC009898 |
| 基因名称 | SMS |
| Gene ID (NCBI) | 6611 |
| RRID | AB_2270820 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P52788 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Spermine synthase antibody 15979-1-AP | Download protocol |
| WB protocol for Spermine synthase antibody 15979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Predicting lung adenocarcinoma prognosis, immune escape, and pharmacomic profile from arginine and proline-related genes | ||
Cells Magnesium Supplementation Attenuates Ultraviolet-B-Induced Damage Mediated through Elevation of Polyamine Production in Human HaCaT Keratinocytes. | ||
Proteomics Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line. | ||
J Proteomics Proteomic analysis revealed different responses to hypergravity of soleus and extensor digitorum longus muscles in mice. | ||
Biol Reprod Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period. | ||
Alzheimers Res Ther Spermidine/spermine-N1-acetyltransferase ablation impacts tauopathy-induced polyamine stress response. |
