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SMPX Polyclonal antibody

SMPX Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15791-1-AP

Print datasheet

Synonyms

small muscle protein, X linked, Small muscular protein, SMPX, SRMX

验证数据展示

  • WB analysis of human skeletal muscle using 15791-1-AP

    WB analysis of human skeletal muscle using 15791-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15791-1-AP (SMPX antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15791-1-AP

    IHC staining of human kidney using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15791-1-AP (SMPX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 15791-1-AP

    IHC staining of human kidney using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15791-1-AP (SMPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15791-1-AP

    WB analysis using 15791-1-AP

    pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15791-1-AP (SMPX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inpig skeletal muscle tissue, human skeletal muscle tissue, human colon tissue, pig colon tissue
Positive IHC detected inhuman kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15791-1-AP targets SMPX in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, pig
Immunogen SMPX fusion protein Ag8497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small muscle protein, X-linked
Synonyms small muscle protein, X linked, Small muscular protein, SMPX, SRMX
Calculated Molecular Weight 88 aa, 10 kDa
Observed Molecular Weight 9 kDa
GenBank Accession NumberBC005948
Gene Symbol SMPX
Gene ID (NCBI) 23676
RRIDAB_2193527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHP9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SMPX antibody 15791-1-APDownload protocol
IHC protocol for SMPX antibody 15791-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols