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验证数据展示

  • WB analysis of human skeletal muscle using 15791-1-AP

    WB analysis of human skeletal muscle using 15791-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15791-1-AP (SMPX antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15791-1-AP

    IHC staining of human kidney using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15791-1-AP (SMPX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 15791-1-AP

    IHC staining of human kidney using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15791-1-AP (SMPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15791-1-AP

    WB analysis using 15791-1-AP

    pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15791-1-AP (SMPX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SMPX Polyclonal antibody

SMPX Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15791-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IHC, ELISA

别名

small muscle protein, X linked, Small muscular protein, SMPX, SRMX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig skeletal muscle tissue, human skeletal muscle tissue, human colon tissue, pig colon tissue
Positive IHC detected inhuman kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15791-1-AP targets SMPX in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag8497

Product name: Recombinant human SMPX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-88 aa of BC005948

Sequence: MNMSKQPVSNVRAIQANINIPMGAFRPGAGQPPRRKECTPEVEEGVPPTSDEEKKPIPGAKKLPGPAVNLSEIQNIKSELKYVPKAEQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small muscle protein, X-linked
别名 small muscle protein, X linked, Small muscular protein, SMPX, SRMX
计算分子量 88 aa, 10 kDa
观测分子量 9 kDa
GenBank蛋白编号BC005948
基因名称 SMPX
Gene ID (NCBI) 23676
RRIDAB_2193527
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHP9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SMPX antibody 15791-1-APDownload protocol
IHC protocol for SMPX antibody 15791-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols