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验证数据展示

  • WB analysis using 25613-1-AP (same clone as 25613-1-PBS)

    WB analysis using 25613-1-AP (same clone as 25613-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25613-1-AP (SMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25613-1-PBS in a different storage buffer formulation.

  • WB analysis using 25613-1-AP (same clone as 25613-1-PBS)

    WB analysis using 25613-1-AP (same clone as 25613-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 25613-1-AP (SMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25613-1-PBS in a different storage buffer formulation.

SMPD3 Polyclonal antibody, PBS Only

SMPD3 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 25613-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

EC:3.1.4.12, Neutral sphingomyelinase 2, Neutral sphingomyelinase II, nSMase 2, nSMase2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25613-1-PBS targets SMPD3 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22487

Product name: Recombinant human SMPD3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 540-655 aa of BC112238

Sequence: PGEEKPWAIGTLLDTNGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSSGQKGRKELLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFITQLSGLTDHLPVAMRLMVSSGEEEA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)
别名 EC:3.1.4.12, Neutral sphingomyelinase 2, Neutral sphingomyelinase II, nSMase 2, nSMase2
计算分子量 655 aa, 71 kDa
观测分子量70-71 kDa
GenBank蛋白编号BC112238
基因名称 SMPD3
Gene ID (NCBI) 55512
RRIDAB_3742154
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NY59
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMPD3 (sphingomyelin phosphodiesterase 3), also called neutral sphingomyelinase-2 (nSMase2), is a Golgi-enriched, membrane-bound Zn²⁺-dependent hydrolase that converts sphingomyelin into ceramide and phosphocholine. Ceramide functions as a bioactive lipid second messenger that integrates cellular stress, apoptosis, autophagy and differentiation signals.

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