• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human placenta using 30197-1-AP

    WB analysis of human placenta using 30197-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30197-1-AP (SMPD1,ASM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 30197-1-AP

    IF Staining of NIH/3T3 using 30197-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using SMPD1,ASM antibody (30197-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

SMPD1,ASM Polyclonal antibody

SMPD1,ASM Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30197-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

SMPD1, Sphingomyelin phosphodiesterase, EC:3.1.4.12, aSMase, ASM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30197-1-AP targets SMPD1,ASM in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SMPD1,ASM fusion protein Ag32479 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sphingomyelin phosphodiesterase 1, acid lysosomal
别名 SMPD1, Sphingomyelin phosphodiesterase, EC:3.1.4.12, aSMase, ASM
计算分子量 70 kDa
观测分子量68-70 kDa
GenBank蛋白编号BC041164
基因名称 SMPD1
Gene ID (NCBI) 6609
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17405
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing. As a catalytically inactive 75kDa precursor, after signal peptide cleavage, it was processed into smaller non-glycosylated and rapidly degraded 57kDa form in ER-Golgi complex. And 70kDa mature form with major catalytic activity located in lysosomes, which is further processed into 52kDa form (PMID:25853898, PMID:26499107).

实验方案

Product Specific Protocols
WB protocol for SMPD1,ASM antibody 30197-1-APDownload protocol
IF protocol for SMPD1,ASM antibody 30197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols