验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, mouse brain tissue, mouse ovary tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31063-1-AP targets SMOC2 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse |
| 免疫原 | SMOC2 fusion protein Ag34966 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SPARC related modular calcium binding 2 |
| 别名 | bA270C4A.1, bA37D8.1, dJ421D16.1, MST117, MSTP117, MSTP140, SMAP 2, SMAP2, SMOC 2, SMOC2 |
| 计算分子量 | 50 kDa |
| 观测分子量 | 49-50 kDa |
| GenBank蛋白编号 | BC047583 |
| 基因名称 | SMOC2 |
| Gene ID (NCBI) | 64094 |
| RRID | AB_3669838 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9H3U7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Secreted modular calcium-binding protein 2 (SMOC2), which is a member of the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins, interacts with matrix proteins, cell surface receptors, cytokines, proteasomes and other effector molecules to regulate cell-cell and cell-microenvironment communication (PMID: 36513634). It is expected to be located in extracellular space. The calculated molecular weight of SMOC2 is 50 kDa. In recent years, SMOC2 has been reported to be highly expressed in tumor tissues and to promote tumor migration, invasion and growth. In addition to its role in tumor progression, it also regulates wound healing, bone growth, fibrosis and embryogenesis (PMID: 36513634).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMOC2 antibody 31063-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |