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验证数据展示

  • WB analysis of mouse heart using 86654-3-RR (same clone as 86654-3-PBS)

    WB analysis of mouse heart using 86654-3-RR (same clone as 86654-3-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 86654-3-RR (MYH11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86654-3-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 86654-3-RR (same clone as 86654-3-PBS)

    IHC staining of human breast cancer using 86654-3-RR (same clone as 86654-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86654-3-RR (SMMHC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86654-3-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 86654-3-RR (same clone as 86654-3-PBS)

    IHC staining of human colon using 86654-3-RR (same clone as 86654-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86654-3-RR (SMMHC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86654-3-PBS in a different storage buffer formulation.

  • Affinity of 86654-3-RR

    Affinity of 86654-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86654-3-RR against Human SMMHC were performed. The affinity constant is 1.48 nM.

SMMHC Recombinant monoclonal antibody, PBS Only

SMMHC Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86654-3-PBS

产品说明书

CloneNo. 251604H2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

MYH11, KIAA0866, Myosin 11, Myosin heavy chain 11, Myosin-11

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86654-3-PBS targets SMMHC in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16114

Product name: Recombinant human MYH11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1208-1316 aa of BC104906

Sequence: LTEQLEQFKRAKANLDKNKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEAQVQELQSKCSDGERARAELNDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLSSQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin, heavy chain 11, smooth muscle
别名 MYH11, KIAA0866, Myosin 11, Myosin heavy chain 11, Myosin-11
计算分子量 1979 aa, 228 kDa
观测分子量227-250 kDa
GenBank蛋白编号BC104906
基因名称 SMMHC/MYH11
Gene ID (NCBI) 4629
RRIDAB_3745033
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35749
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.

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