验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse kidney tissue, mouse testis tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 1 publications below |
产品信息
27849-1-AP targets SMIM1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | Human, Mouse |
文献引用反应性 | human |
免疫原 | SMIM1 fusion protein Ag27028 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | hypothetical LOC388588 |
别名 | hypothetical LOC388588, LOC388588, Small integral membrane protein 1, SMIM1 |
观测分子量 | 9 kDa |
GenBank蛋白编号 | BC146957 |
基因名称 | SMIM1 |
Gene ID (NCBI) | 388588 |
RRID | AB_2880993 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | B2RUZ4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Small integral membrane protein 1(SMIM1) is a type II transmembrane phosphoprotein and displays the Vel blood group antigen at its carboxyl-terminus. SMIM1 is highly expressed in hematopoietic cells. It is associated with a variety of immune responses and plays an important role in RBC differentiation. SMIM1 can be used as a potential biomarker for the diagnosis of IDD (PMID: 30906890; 26452714).
实验方案
Product Specific Protocols | |
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WB protocol for SMIM1 antibody 27849-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |