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验证数据展示

  • IHC staining of mouse heart using 12694-1-AP (same clone as 12694-1-PBS)

    IHC staining of mouse heart using 12694-1-AP (same clone as 12694-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12694-1-AP (SMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12694-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 12694-1-AP (same clone as 12694-1-PBS)

    IF Staining of MCF-7 using 12694-1-AP (same clone as 12694-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMG5 antibody (12694-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12694-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 12694-1-AP (same clone as 12694-1-PBS)

    IF Staining of Jurkat using 12694-1-AP (same clone as 12694-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SMG5 antibody (12694-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12694-1-PBS in a different storage buffer formulation.

SMG5 Polyclonal antibody, PBS Only

SMG5 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 12694-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

EST1 like protein B, EST1B, EST1-like protein B, hSMG 5, hSMG-5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12694-1-PBS targets SMG5 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3424

Product name: Recombinant human SMG5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 670-1016 aa of BC038296

Sequence: DLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKDVLDFYKQWKEIG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans)
别名 EST1 like protein B, EST1B, EST1-like protein B, hSMG 5, hSMG-5
计算分子量 1016 aa, 114 kDa
GenBank蛋白编号BC038296
基因名称 SMG5
Gene ID (NCBI) 23381
RRIDAB_2270781
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPR3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMG5 is also named EST1-like protein B (EST1B) and LPTS-RP1. SMG5 is a key Nonsense-mediated mRNA decay (NMD) factor, and NMD factor SMG5 is vital for embryonic development (PMID: 39199410). SMG5 plays a role in the degradation of mRNA through the process of nonsense-mediated decay and encodes a protein. SMG5 has been linked to the development of pancreatic cancer and epiphyseal chondrodysplasia among various illnesses (PMID: 36056355). SMG5 is a kind of RNA-binding proteins (RBPs) (PMID: 37936239). In addition, they have been shown to be key regulators of oncogenesis and tumor progression (PMID: 33228611). SMG5 may be a high-risk factor for HCC prognosis (PMID: 33411682). SMG5 promotes HCC cell proliferation and tumor growth. SMG5 has extensive effects on the proliferation, survival, and tumor growth of HCC cells.

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