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验证数据展示

  • WB analysis of HEK-293 using 25815-1-AP

    WB analysis of HEK-293 using 25815-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25815-1-AP (SMCO4 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 25815-1-AP

    IHC staining of human colon cancer using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 25815-1-AP

    IHC staining of human colon cancer using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SMCO4 Polyclonal antibody

SMCO4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25815-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C11orf75, UPF0443 protein C11orf75, Single-pass membrane and coiled-coil domain-containing protein 4, Protein FN5, FN5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25815-1-AP targets SMCO4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 SMCO4 fusion protein Ag22806 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 75
别名 C11orf75, UPF0443 protein C11orf75, Single-pass membrane and coiled-coil domain-containing protein 4, Protein FN5, FN5
计算分子量 59 aa, 7 kDa
观测分子量 7 kDa
GenBank蛋白编号BC031564
基因名称 SMCO4
Gene ID (NCBI) 56935
RRIDAB_2880249
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRQ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMCO4, also named as C11orf75 and FN5, is a Single-pass membrane and coiled-coil domain-containing protein.

实验方案

Product Specific Protocols
WB protocol for SMCO4 antibody 25815-1-APDownload protocol
IHC protocol for SMCO4 antibody 25815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols