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SMCO4 Polyclonal antibody

SMCO4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25815-1-AP

Print datasheet

Synonyms

C11orf75, FN5, Protein FN5, UPF0443 protein C11orf75

验证数据展示

  • WB analysis of HEK-293 using 25815-1-AP

    WB analysis of HEK-293 using 25815-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25815-1-AP (SMCO4 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 25815-1-AP

    IHC staining of human colon cancer using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 40x lens).

  • IHC staining of human colon cancer using 25815-1-AP

    IHC staining of human colon cancer using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 25815-1-AP

    IHC staining of human colon cancer using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25815-1-AP is SMCO4 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen SMCO4 fusion protein Ag22806 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 11 open reading frame 75
Synonyms C11orf75, FN5, Protein FN5, UPF0443 protein C11orf75
Calculated Molecular Weight 59 aa, 7 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC031564
Gene Symbol C11orf75
Gene ID (NCBI) 56935
RRIDAB_2880249
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRQ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMCO4, also named as C11orf75 and FN5, is a Single-pass membrane and coiled-coil domain-containing protein.

实验方案

Product Specific Protocols
WB protocol for SMCO4 antibody 25815-1-APDownload protocol
IHC protocol for SMCO4 antibody 25815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols