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  • Featured Product
  • KD/KO Validated

SMC5 Polyclonal antibody

SMC5 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14178-1-AP

Print datasheet

Synonyms

hSMC5, KIAA0594, SMC 5, SMC protein 5, SMC5, SMC5L1

验证数据展示

  • WB analysis of HeLa using 14178-1-AP

    WB analysis of HeLa using 14178-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14178-1-AP (SMC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14178-1-AP

    IF Staining of HeLa using 14178-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14178-1-AP (SMC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14178-1-AP targets SMC5 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SMC5 fusion protein Ag5385 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name structural maintenance of chromosomes 5
Synonyms hSMC5, KIAA0594, SMC 5, SMC protein 5, SMC5, SMC5L1
Calculated Molecular Weight 1101 aa, 129 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC038225
Gene Symbol SMC5
Gene ID (NCBI) 23137
RRIDAB_2192775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IY18
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SMC5 antibody 14178-1-APDownload protocol
IF protocol for SMC5 antibody 14178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

mSystems

Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes

Authors - Joseph M. Dybas
  • KD Validated
View Article
humanIF

J Virol

PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner.

Authors - Wei Xu
View Article
humanWB

Cell Rep

Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.

Authors - Stephanie Pei Tung Yiu
View Article