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验证数据展示

  • WB analysis of human liver using 16597-1-AP

    WB analysis of human liver using 16597-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16597-1-AP (SMAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16597-1-AP

    IHC staining of human liver using 16597-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16597-1-AP (SMAP2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 16597-1-AP

    IHC staining of human liver using 16597-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16597-1-AP (SMAP2 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 16597-1-AP

    WB analysis using 16597-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16597-1-AP (SMAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16597-1-AP

    IHC staining of human lung cancer using 16597-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16597-1-AP (SMAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 16597-1-AP

    IF Staining of HepG2 using 16597-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SMAP2 antibody (16597-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SMAP2 Polyclonal antibody

SMAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16597-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

small ArfGAP2, SMAP1L, Stromal membrane-associated protein 1-like, Stromal membrane-associated protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman liver tissue, human colon tissue, K-562 cells, mouse small intestine tissue, LNCaP cells, PC-3 cells
Positive IHC detected inhuman liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16597-1-AP targets SMAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag9751

Product name: Recombinant human SMAP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 77-429 aa of BC021133

Sequence: QIQCMQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINAFRKEKDDKWKRGSEPVPEKKLEPVVFEKVKMPQKKEDPQLPRKSSPKSTAPVMDLLGLDAPVACSIANSKTSNTLEKDLDLLASVPSPSSSGSRKVVGSMPTAGSAGSVPENLNLFPEPGSKSEEIGKKQLSKDSILSLYGSQTPQMPTQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGSMMPPPVGMVAQPGASGMVAPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMAGMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMSGGNGQAANQTLSPQMWK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small ArfGAP2
别名 small ArfGAP2, SMAP1L, Stromal membrane-associated protein 1-like, Stromal membrane-associated protein 2
计算分子量 429 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC021133
基因名称 SMAP2
Gene ID (NCBI) 64744
RRIDAB_2066026
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WU79
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SMAP2 antibody 16597-1-APDownload protocol
IHC protocol for SMAP2 antibody 16597-1-APDownload protocol
WB protocol for SMAP2 antibody 16597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice.

Authors - Chuan Wang
View Article
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