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验证数据展示

  • WB analysis of human liver using 16597-1-AP

    WB analysis of human liver using 16597-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16597-1-AP (SMAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16597-1-AP

    IHC staining of human liver using 16597-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16597-1-AP (SMAP2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 16597-1-AP

    IHC staining of human liver using 16597-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16597-1-AP (SMAP2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung cancer using 16597-1-AP

    IHC staining of human lung cancer using 16597-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16597-1-AP (SMAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 16597-1-AP

    IF Staining of HepG2 using 16597-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SMAP2 antibody (16597-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SMAP2 Polyclonal antibody

SMAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16597-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

small ArfGAP2, SMAP1L, Stromal membrane-associated protein 1-like, Stromal membrane-associated protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman liver tissue, human colon tissue, mouse small intestine tissue
Positive IHC detected inhuman liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16597-1-AP targets SMAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9751

Product name: Recombinant human SMAP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 77-429 aa of BC021133

Sequence: QIQCMQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINAFRKEKDDKWKRGSEPVPEKKLEPVVFEKVKMPQKKEDPQLPRKSSPKSTAPVMDLLGLDAPVACSIANSKTSNTLEKDLDLLASVPSPSSSGSRKVVGSMPTAGSAGSVPENLNLFPEPGSKSEEIGKKQLSKDSILSLYGSQTPQMPTQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGSMMPPPVGMVAQPGASGMVAPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMAGMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMSGGNGQAANQTLSPQMWK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small ArfGAP2
别名 small ArfGAP2, SMAP1L, Stromal membrane-associated protein 1-like, Stromal membrane-associated protein 2
计算分子量 429 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC021133
基因名称 SMAP2
Gene ID (NCBI) 64744
RRIDAB_2066026
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WU79
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SMAP2 antibody 16597-1-APDownload protocol
IHC protocol for SMAP2 antibody 16597-1-APDownload protocol
IF protocol for SMAP2 antibody 16597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols