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验证数据展示

  • WB analysis using 30910-1-AP

    WB analysis using 30910-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30910-1-AP (LOC57228 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 30910-1-AP

    WB analysis of HeLa using 30910-1-AP

    WB result of SMAGP antibody (30910-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAGP transfected HeLa cells.

  • IF Staining of HeLa using 30910-1-AP

    IF Staining of HeLa using 30910-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LOC57228 antibody (30910-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO验证

SMAGP Polyclonal antibody

SMAGP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30910-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

LOC57228

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse colon tissue, mouse lung tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30910-1-AP targets SMAGP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SMAGP fusion protein Ag34278 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small trans-membrane and glycosylated protein
别名 LOC57228
观测分子量27-30 kDa
GenBank蛋白编号BC120965
基因名称 SMAGP
Gene ID (NCBI) 57228
RRIDAB_3669781
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ0VAQ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small transmembrane and glycosylated protein (SMAGP) consists of 97 amino acids and contains a transmembrane domain. SMAGP localizes to the lateral face of the plasma membrane and is expressed in the cytoplasm after cell transformation. (PMID: 30410350)

实验方案

Product Specific Protocols
WB protocol for SMAGP antibody 30910-1-APDownload protocol
IF protocol for SMAGP antibody 30910-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols