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验证数据展示

  • WB analysis using 80964-5-RR (same clone as 80964-5-PBS)

    WB analysis using 80964-5-RR (same clone as 80964-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80964-5-RR (SMAD7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80964-5-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 80964-5-RR (same clone as 80964-5-PBS)

    IHC staining of human urothelial carcinoma using 80964-5-RR (same clone as 80964-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 80964-5-RR (SMAD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80964-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD7 antibody included
  • IHC staining of mouse liver using 80964-5-RR (same clone as 80964-5-PBS)

    IHC staining of mouse liver using 80964-5-RR (same clone as 80964-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 80964-5-RR (SMAD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80964-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD7 antibody included
  • IHC staining of mouse liver using 80964-5-RR (same clone as 80964-5-PBS)

    IHC staining of mouse liver using 80964-5-RR (same clone as 80964-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 80964-5-RR (SMAD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80964-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD7 antibody included

SMAD7 Recombinant monoclonal antibody, PBS Only

SMAD7 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80964-5-PBS

产品说明书

CloneNo. 251688E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

CRCS3, hSMAD7, MAD homolog 7, MAD homolog 8, MADH7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® SMAD7 Ready-To-Use IHC Kit. SMAD7 primary antibody included.

产品信息

80964-5-PBS targets SMAD7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13688

Product name: Recombinant human SMAD7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-426 aa of BC074819

Sequence: MFRTKRSALVRRLWRSRAPGGEDEEEGAGGGGGGGELRGEGATDSRAHGAGGGGPGRAGCCLGKAVRGAKGHHHPHPPAAGAGAAGGAEADLKALTHSVLKKLKERQLELLLQAVESRGGTRTACLLLPGRLDCRLGPGAPAGAQPAQPPSSYSLPLLLCKVFRWPDLRHSSEVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSRYPMDFLKPTADCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSHWCVVAYWEEKTRVGRLYCVQEPSLDIFYDLPQGNGFCLGQLNSDNKSQLVQKVRSKIGCGIQLTREVDGVWVYNRSSYPIFIKSATLDNPDSRTLLVHKVFPGFSIKAFDYEKAYSLQRPNDHEFMQQPWTGFTVQISFVKGWGQCYTRQFISSCPCWLEVIFNSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SMAD family member 7
别名 CRCS3, hSMAD7, MAD homolog 7, MAD homolog 8, MADH7
计算分子量 426 aa, 46 kDa
观测分子量58 kDa
GenBank蛋白编号BC074819
基因名称 SMAD7
Gene ID (NCBI) 4092
RRIDAB_3743361
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15105
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta type 1 receptor superfamily members has been shown to inhibit TGF-beta and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

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