验证数据展示

WB analysis using 10231-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 10231-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10231-1-AP
IP result of anti-SMAD4 (IP:10231-1-AP, 4ug; Detection:10231-1-AP 1:1500) with HeLa cells lysate 1320 ug.
WB analysis using 10231-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human skin cancer using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SMAD4 antibody included
IHC staining of mouse pancreas using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SMAD4 antibody included
IHC staining of human cervical cancer using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SMAD4 antibody included
IHC staining of rat small intestine using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SMAD4 antibody included

Featured Product
KD/KO Validated

SMAD4 Polyclonal antibody

SMAD4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10231-1-AP

产品说明书

引用文献(139)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (5)

应用

WB, IHC, IP, ELISA and More (3)

别名

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (139)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293T cells, COLO 320 cells, HCT 116 cells, HEK-293 cells, mouse liver tissue, NIH/3T3 cells, Neuro-2a cells, C6 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human skin cancer tissue, human tonsillitis tissue, human cervical cancer tissue, mouse pancreas tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 6 publications below
WB	See 111 publications below
IHC	See 17 publications below
IF	See 13 publications below
IP	See 6 publications below
CoIP	See 4 publications below
ChIP	See 9 publications below

产品信息

10231-1-AP targets SMAD4 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, ChIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, bovine, hamster, sheep, goat
免疫原	CatNo: Ag0299 Product name: Recombinant human SMAD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 13-227 aa of BC002379 Sequence: NDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPAS 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	SMAD family member 4
别名	DPC4, hSMAD4, JIP, MAD homolog 4, MADH4
计算分子量	60 kDa
观测分子量	63-70 kDa
GenBank蛋白编号	BC002379
基因名称	SMAD4
Gene ID (NCBI)	4089
RRID	AB_2193323
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q13485
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against an internal region of human SMAD4.

实验方案

Product Specific Protocols
IHC protocol for SMAD4 antibody 10231-1-AP	Download protocol
IP protocol for SMAD4 antibody 10231-1-AP	Download protocol
WB protocol for SMAD4 antibody 10231-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB,IP,IF,ChIP	Signal Transduct Target Ther Annexin A1 binds PDZ and LIM domain 7 to inhibit adipogenesis and prevent obesity Authors - Lu Fang View Article
mouse	WB	Nature Post-translational control of beige fat biogenesis by PRDM16 stabilization Authors - Qiang Wang View Article
human	WB,IF	Mol Cancer HDAC6 orchestrates metastatic and immunosuppressive programs in small cell lung cancer through S100A2-TGF-β/SMAD and CSF1R signaling. Authors - Yantao Jiang View Article
mouse	WB	Cancer Res MPND Loss Epigenetically Activates TGF-β/SMAD3 Signaling to Drive Tumor Progression and Metastasis in Non-small Cell Lung Cancer. Authors - Jianfeng Yu View Article
		Nat Commun A Rras2-BMPR2 feedback loop sustains osteogenesis and represents a therapeutic target for osteoporosis. Authors - Renlei Yang View Article
mouse,human	WB,IHC,ChIP	Nat Commun Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling Authors - Jinjin Yu View Article

验证数据展示

WB analysis using 10231-1-AP

WB analysis using 10231-1-AP

IP experiment of HeLa using 10231-1-AP

WB analysis using 10231-1-AP

IHC staining of human skin cancer using 10231-1-AP

IHC staining of mouse pancreas using 10231-1-AP

IHC staining of human cervical cancer using 10231-1-AP

IHC staining of rat small intestine using 10231-1-AP

SMAD4 Polyclonal antibody

SMAD4 Polyclonal Antibody for WB, IHC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Annexin A1 binds PDZ and LIM domain 7 to inhibit adipogenesis and prevent obesity

Post-translational control of beige fat biogenesis by PRDM16 stabilization

HDAC6 orchestrates metastatic and immunosuppressive programs in small cell lung cancer through S100A2-TGF-β/SMAD and CSF1R signaling.

MPND Loss Epigenetically Activates TGF-β/SMAD3 Signaling to Drive Tumor Progression and Metastasis in Non-small Cell Lung Cancer.

A Rras2-BMPR2 feedback loop sustains osteogenesis and represents a therapeutic target for osteoporosis.

Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling

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