验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, NIH/3T3 cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells |
| Positive IHC detected in | human stomach cancer tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SMAD3 Ready-To-Use IHC Kit. SMAD3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66516-1-Ig targets SMAD3 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig, rabbit |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SMAD family member 3 |
| 别名 | JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | NM_005902 |
| 基因名称 | SMAD3 |
| Gene ID (NCBI) | 4088 |
| RRID | AB_2881879 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P84022 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SMAD3 antibody 66516-1-Ig | Download protocol |
| WB protocol for SMAD3 antibody 66516-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer HDAC6 orchestrates metastatic and immunosuppressive programs in small cell lung cancer through S100A2-TGF-β/SMAD and CSF1R signaling. | ||
Bioact Mater Hydrogel delivering antifibrotic agent and nano-sonosensitizer enhances efficacy of sonodynamic therapy in osteosarcoma treatment. | ||
Bioact Mater Stage-aligned electro-ionic biobattery hydrogel for enhanced wound closure and scar reduction. | ||
Nat Commun Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling | ||
J Adv Res SIRT7 facilitates ferroptosis resistance of melanocytes via activating the SMAD3-ATF3-GPX4 signaling pathway in vitiligo | ||
J Nanobiotechnology Modification of transvaginal polypropylene mesh with co-axis electrospun nanofibrous membrane to alleviate complications following surgical implantation |
