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验证数据展示

  • WB analysis using 30130-1-AP

    WB analysis using 30130-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30130-1-AP (SMAD3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 30130-1-AP

    IHC staining of human stomach cancer using 30130-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMAD3 antibody included
  • IHC staining of human stomach cancer using 30130-1-AP

    IHC staining of human stomach cancer using 30130-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMAD3 antibody included

SMAD3 Polyclonal antibody

SMAD3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30130-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SMAD3 Ready-To-Use IHC Kit. SMAD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30130-1-AP targets SMAD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 SMAD3 fusion protein Ag32595 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SMAD family member 3
别名 JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3
计算分子量48kd
观测分子量48-55 kDa
GenBank蛋白编号NM_005902
基因名称 SMAD3
Gene ID (NCBI) 4088
RRIDAB_3662144
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP84022
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

实验方案

Product Specific Protocols
WB protocol for SMAD3 antibody 30130-1-APDownload protocol
IHC protocol for SMAD3 antibody 30130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Clin Endocrinol Metab

CD34+ orbital fibroblasts contribute to the pathogenesis of thyroid eye disease via miR-182-5p

Authors - Baiguang Yu
View Article