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验证数据展示

  • WB analysis of mouse brain using 17889-1-AP

    WB analysis of mouse brain using 17889-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 17889-1-AP

    IHC staining of human skin cancer using 17889-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17889-1-AP (SLTM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLTM antibody included
  • IF Staining of HepG2 using 17889-1-AP

    IF Staining of HepG2 using 17889-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLTM antibody (17889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SLTM Polyclonal antibody

SLTM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17889-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

SAFB-like transcription modulator, Modulator of estrogen-induced transcription, MET

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SLTM Ready-To-Use IHC Kit. SLTM primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17889-1-AP targets SLTM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLTM fusion protein Ag12060 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SAFB-like, transcription modulator
别名 SAFB-like transcription modulator, Modulator of estrogen-induced transcription, MET
计算分子量478aa,57 kDa; 1034aa,117 kDa
观测分子量 135-140 kDa
GenBank蛋白编号BC046119
基因名称 SLTM
Gene ID (NCBI) 79811
RRIDAB_2192729
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWH9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLTM, also named as Modulator of estrogen-induced transcription, is a 1034 amino acid protein, which localizes in the nucleus. When it is overexpressed, SLTM acts as a general inhibitor of transcription that eventually leads to apoptosis. The calculated molecular weight of SLTM is 117 kDa, but modified SLTM is about 135-140 kDa.

实验方案

Product Specific Protocols
WB protocol for SLTM antibody 17889-1-APDownload protocol
IHC protocol for SLTM antibody 17889-1-APDownload protocol
IF protocol for SLTM antibody 17889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols