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验证数据展示

  • WB analysis of mouse brain using 21649-1-AP

    WB analysis of mouse brain using 21649-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21649-1-AP

    IP experiment of mouse brain using 21649-1-AP

    IP result of anti-SLITRK3 (IP:21649-1-AP, 4ug; Detection:21649-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • IHC staining of mouse brain using 21649-1-AP

    IHC staining of mouse brain using 21649-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse intestine using 21649-1-AP

    IHC staining of mouse intestine using 21649-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse intestine using 21649-1-AP

    IHC staining of mouse intestine using 21649-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat small intestine using 21649-1-AP

    IHC staining of rat small intestine using 21649-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat small intestine using 21649-1-AP

    IHC staining of rat small intestine using 21649-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

SLITRK3 Polyclonal antibody

SLITRK3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 21649-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

别名

SLIT and NTRK-like protein 3, KIAA0848

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat small intestine tissue, mouse brain tissue, mouse intestine
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21649-1-AP targets SLITRK3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag16352

Product name: Recombinant human SLITRK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 745-964 aa of BC114621

Sequence: HPVTQMCNNPIYKPREEEEVAVSSAQEAGSAERGGPGTQPPGMGEALLGSEQFAETPKENHSNYRTLLEKEKEWALAVSSSQLNTIVTVNHHHPHHPAVGGVSGVVGGTGGDLAGFRHHEKNGGVVLFPPGGGCGSGSMLLDRERPQPAPCTVGFVDCLYGTVPKLKELHVHPPGMQYPDLQQDARLKETLLFSAGKGFTDHQTQKSDYLELRAKLQTKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SLIT and NTRK-like family, member 3
别名 SLIT and NTRK-like protein 3, KIAA0848
计算分子量 977 aa, 109 kDa
观测分子量 120-125 kDa
GenBank蛋白编号BC114621
基因名称 SLITRK3
Gene ID (NCBI) 22865
RRIDAB_10859788
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94933
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SLITRK3 antibody 21649-1-APDownload protocol
IP protocol for SLITRK3 antibody 21649-1-APDownload protocol
WB protocol for SLITRK3 antibody 21649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB, IHC

Neuron

Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.

Authors - Jun Li
View Article
ratWB

Proc Natl Acad Sci U S A

Slitrks control excitatory and inhibitory synapse formation with LAR receptor protein tyrosine phosphatases.

Authors - Yim Yeong Shin YS
  • KD Validated
View Article
humanWB,IF

Mol Biomed

Role of novel cancer gene SLITRK3 to activate NTRK3 in squamous cell lung cancer.

Authors - Aliccia Bollig-Fischer
  • KD Validated
View Article
humanWB

Front Mol Neurosci

Human mutations in SLITRK3 implicated in GABAergic synapse development in mice

Authors - Stephanie Efthymiou
View Article
mouseWB

J Neurosci

A critical role of Neuroligin 2 C-terminus in OCD and social behavior

Authors - Saurabh Pandey
View Article
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