SLIT2 Polyclonal antibody

SLIT2 Polyclonal Antibody for WB, ELISA
Cat No. 28730-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

Slit-2, Slit homolog 2 protein N-product, Slit homolog 2 protein C-product, Slit homolog 2 protein, Slit 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28730-1-AP targets SLIT2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 SLIT2 fusion protein Ag30428 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 slit homolog 2 (Drosophila)
别名 Slit-2, Slit homolog 2 protein N-product, Slit homolog 2 protein C-product, Slit homolog 2 protein, Slit 2
计算分子量 170 kDa
观测分子量100 kDa, 200 kDa
GenBank蛋白编号NM_004787
基因名称 SLIT2
Gene ID (NCBI) 9353
RRIDAB_2935470
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLIT2, also named as SLIL3, is thought to act as a molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development is involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, SLIT2 may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. SLIT2 may be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system it appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, it collapses and repels retinal ganglion cell growth cones. SLIT2 seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. It seems to be involved in regulating leukocyte migration. The antibody is specific to SLIT2. Slit2 is cleaved into 140 kDa N-terminal (Slit2-N) and 55-60 kDa C-terminal (Slit2-C) fragments, although the uncleaved/full-length form(200) can also be detected.

实验方案

Product Specific Protocols
WB protocol for SLIT2 antibody 28730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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