验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28730-1-AP targets SLIT2 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | SLIT2 fusion protein Ag30428 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | slit homolog 2 (Drosophila) |
别名 | Slit-2, Slit homolog 2 protein N-product, Slit homolog 2 protein C-product, Slit homolog 2 protein, Slit 2 |
计算分子量 | 170 kDa |
观测分子量 | 100 kDa, 200 kDa |
GenBank蛋白编号 | NM_004787 |
基因名称 | SLIT2 |
Gene ID (NCBI) | 9353 |
RRID | AB_2935470 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O94813 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLIT2, also named as SLIL3, is thought to act as a molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development is involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, SLIT2 may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. SLIT2 may be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system it appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, it collapses and repels retinal ganglion cell growth cones. SLIT2 seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. It seems to be involved in regulating leukocyte migration. The antibody is specific to SLIT2. Slit2 is cleaved into 140 kDa N-terminal (Slit2-N) and 55-60 kDa C-terminal (Slit2-C) fragments, although the uncleaved/full-length form(200) can also be detected.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for SLIT2 antibody 28730-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |