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验证数据展示

  • WB analysis using 30550-1-AP

    WB analysis using 30550-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30550-1-AP (SLFN12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse placenta using 30550-1-AP

    IHC staining of mouse placenta using 30550-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30550-1-AP (SLFN12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse placenta using 30550-1-AP

    IHC staining of mouse placenta using 30550-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30550-1-AP (SLFN12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLFN12 Polyclonal antibody

SLFN12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30550-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

EC:3.1.-.-, Ribonuclease SLFN12, Schlafen family member 12, SLFN3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDU 145 cells, HEK-293 cells, HeLa cells, U2OS cells
Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30550-1-AP targets SLFN12 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31289

Product name: Recombinant human SLFN12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 340-402 aa of BC035605

Sequence: FMVEAEPKFSSSYEEVISQINTSLPAPHSWPLLEWQRQRHHCPGLSGRITYTPENLCRKLFLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 schlafen family member 12
别名 EC:3.1.-.-, Ribonuclease SLFN12, Schlafen family member 12, SLFN3
计算分子量 578 aa, 67 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC035605
基因名称 SLFN12
Gene ID (NCBI) 55106
RRIDAB_3742351
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYM2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Schlafen family member 12 (SLFN12) is the only intermediate Schlafen protein expressed in humans (PMID: 31838790, PMID: 34571887). SLFN12 stimulates differentiation in enterocytes and prostate cancer cells, and its overexpression inhibits prostate cancer, triple-negative breast cancer (TNBC), and lung adenocarcinoma cell proliferation (PMID: 30045019, PMID: 24768141, PMID: 39594803). SLFN12 regulates human enterocytic differentiation by a pathway involving SERPB12, the deubiquitylases, and Cdx2 (PMID: 30045019). The expression of SLFN12 is increased throughout the process of T-cell activation (PMID: 28460425).

实验方案

Product Specific Protocols
IHC protocol for SLFN12 antibody 30550-1-APDownload protocol
WB protocol for SLFN12 antibody 30550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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