验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| CoIP | See 1 publications below |
产品信息
13718-1-AP targets SLC9A9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4660 Product name: Recombinant human SLC9A9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 481-645 aa of BC035779 Sequence: TPMLTWLQIRVGVDLDENLKEDPSSQHQEANNLDKNMTKAESARLFRMWYSFDHKYLKPILTHSGPPLTTTLPEWCGPISRLLTSPQAYGEQLKEDDVECIVNQDELAINYQEQASSPCSPPARLGLDQKASPQTPGKENIYEGDLGLGGYELKLEQTLGQSQLN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 9 (sodium/hydrogen exchanger), member 9 |
| 别名 | NHE-9, NHE9, NHE 9, Nbla00118, Na(+)/H(+) exchanger 9 |
| 计算分子量 | 645 aa, 73 kDa |
| 观测分子量 | 66-73 kDa |
| GenBank蛋白编号 | BC035779 |
| 基因名称 | SLC9A9 |
| Gene ID (NCBI) | 285195 |
| RRID | AB_2189452 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVB4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SLC9A9 antibody 13718-1-AP | Download protocol |
| IHC protocol for SLC9A9 antibody 13718-1-AP | Download protocol |
| WB protocol for SLC9A9 antibody 13718-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Commun Signal MicroRNA-135a regulates NHE9 to inhibit proliferation and migration of glioblastoma cells. | ||
Am J Med Genet B Neuropsychiatr Genet Autism spectrum disorder traits in Slc9a9 knock-out mice. | ||
Atten Defic Hyperact Disord Effect of disease-associated SLC9A9 mutations on protein-protein interaction networks: implications for molecular mechanisms for ADHD and autism. | ||
Mol Cancer Res An Endosomal Acid-Regulatory Feedback System Rewires Cytosolic cAMP Metabolism and Drives Tumor Progression |
