验证数据展示
经过测试的应用
Positive WB detected in | Raji cells, HeLa cells, HepG2 cells |
Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:200-1:600 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
CoIP | See 1 publications below |
产品信息
13718-1-AP targets SLC9A9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, CoIP |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | SLC9A9 fusion protein Ag4660 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | solute carrier family 9 (sodium/hydrogen exchanger), member 9 |
别名 | NHE-9, NHE9, NHE 9, Nbla00118, Na(+)/H(+) exchanger 9 |
计算分子量 | 645 aa, 73 kDa |
观测分子量 | 66-73 kDa |
GenBank蛋白编号 | BC035779 |
基因名称 | SLC9A9 |
Gene ID (NCBI) | 285195 |
RRID | AB_2189452 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8IVB4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for SLC9A9 antibody 13718-1-AP | Download protocol |
IHC protocol for SLC9A9 antibody 13718-1-AP | Download protocol |
IF protocol for SLC9A9 antibody 13718-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Commun Signal MicroRNA-135a regulates NHE9 to inhibit proliferation and migration of glioblastoma cells. | ||
Am J Med Genet B Neuropsychiatr Genet Autism spectrum disorder traits in Slc9a9 knock-out mice. | ||
Atten Defic Hyperact Disord Effect of disease-associated SLC9A9 mutations on protein-protein interaction networks: implications for molecular mechanisms for ADHD and autism. | ||
Mol Cancer Res An Endosomal Acid-Regulatory Feedback System Rewires Cytosolic cAMP Metabolism and Drives Tumor Progression |