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  • KD/KO Validated

CAT-1 Polyclonal antibody

CAT-1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 14195-1-AP

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Synonyms

Amino acid transporter cationic 1, ATRC1, CAT 1, CAT1, CAT-1, HCAT1, REC1L, SLC7A1



经过测试的应用

Positive WB detected inmouse liver tissue, Jurkat cells, HepG2 cells, rat brain/liver tissue, mouse heart tissue, rat liver tissue
Positive IHC detected inhuman osteosarcoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14195-1-AP targets CAT-1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Immunogen CAT-1 fusion protein Ag5398 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
Synonyms Amino acid transporter cationic 1, ATRC1, CAT 1, CAT1, CAT-1, HCAT1, REC1L, SLC7A1
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC063303
Gene Symbol SLC7A1
Gene ID (NCBI) 6541
RRIDAB_2190723
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30825
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAT-1 antibody 14195-1-APDownload protocol
IHC protocol for CAT-1 antibody 14195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.

Authors - Hongling Huang
  • KO Validated
mouseIHC

J Cachexia Sarcopenia Muscle

Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction.

Authors - Thomas Kunzke
mouse, humanWB

J Steroid Biochem Mol Biol

High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.

Authors - Rui Wang
humanWB

Mol Cell Proteomics

Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression.

Authors - James R Byrnes
humanIHC

Front Oncol

An Inflammatory Response-Related Gene Signature Can Impact the Immune Status and Predict the Prognosis of Hepatocellular Carcinoma.

Authors - Zhuo Lin
ratIHC

Exp Neurol

Pseudotype-dependent lentiviral transduction of astrocytes or neurons in the rat substantia nigra.

Authors - Cannon Jason R JR