验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, Jurkat cells, HepG2 cells, rat brain/liver tissue, mouse heart tissue, rat liver tissue |
| Positive IHC detected in | human osteosarcoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14195-1-AP targets SLC7A1/CAT-1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, canine |
| 免疫原 |
CatNo: Ag5398 Product name: Recombinant human SLC7A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 312-629 aa of BC063303 Sequence: MPYFCLDNNSPLPDAFKHVGWEGAKYAVAVGSLCALSASLLGSMFPMPRVIYAMAEDGLLFKFLANVNDRTKTPIIATLASGAVAAVMAFLFDLKDLVDLMSIGTLLAYSLVAACVLVLRYQPEQPNLVYQMASTSDELDPADQNELASTNDSQLGFLPEAEMFSLKTILSPKNMEPSKISGLIVNISTSLIAVLIITFCIVTVLGREALTKGALWAVFLLAGSALLCAVVTGVIWRQPESKTKLSFKVPFLPVLPILSIFVNVYLMMQLDQGTWVRFAVWMLIGFIIYFGYGLWHSEEASLDADQARTPDGNLDQCK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 |
| 别名 | CAT-1, SLC7A1, CAT1, ERR |
| 计算分子量 | 68 kDa |
| 观测分子量 | 67-70 kDa |
| GenBank蛋白编号 | BC063303 |
| 基因名称 | CAT-1 |
| Gene ID (NCBI) | 6541 |
| RRID | AB_2190723 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30825 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC7A1/CAT-1 antibody 14195-1-AP | Download protocol |
| IHC protocol for SLC7A1/CAT-1 antibody 14195-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.
| ||
J Cachexia Sarcopenia Muscle Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction. | ||
Free Radic Biol Med Cold plasma-treated medium preferentially eliminates doxorubicin-resistant osteosarcoma cells | ||
Oxid Med Cell Longev l-Theanine Ameliorates d-Galactose-Induced Brain Damage in Rats via Inhibiting AGE Formation and Regulating Sirtuin1 and BDNF Signaling Pathways. | ||
J Steroid Biochem Mol Biol High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1. | ||
Mol Cell Proteomics Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression. |
