验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30971-1-AP targets SLC6A9 / GlyT1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34437 Product name: Recombinant human SLC6A9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 209-285 aa of NM_201649 Sequence: SSMTHVLPWAYCNNPWNTHDCAGVLDASNLTNGSRPAALPSNLSHLLNHSLQRTSPSEEYWRLYVLKLSDDIGNFGE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 6 (neurotransmitter transporter, glycine), member 9 |
| 别名 | GlyT1, GlyT-1, SLC6A9, Sodium- and chloride-dependent glycine transporter 1, Solute carrier family 6 member 9 |
| 计算分子量 | 706 aa, 78 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | NM_201649 |
| 基因名称 | SLC6A9 |
| Gene ID (NCBI) | 6536 |
| RRID | AB_3669799 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P48067 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC6A9, also named glycine transporter 1 (GlyT1), is a transporter protein of the SLC6 family of sodium- and chloride-dependent neurotransmitter transporters. GlyT1 is the main regulator of synaptic glycine concentrations, assisted by the neuronal GlyT2 (PMID: 28828604). The 55-60 kDa band is the partially glycosylated form of GlyT1 (PMID: 27874011, PMID: 8995423).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC6A9 / GlyT1 antibody 30971-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
