验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue |
| Positive IHC detected in | rat eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26725-1-AP targets SLC6A6/TAUT in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag24935 Product name: Recombinant human SLC6A6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 564-620 aa of BC137128 Sequence: RLCQTEGPFLVRVKYLLTPREPNRWAVEREGATPYNSRTVMNGALVKPTHIIVETMM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 6 (neurotransmitter transporter, taurine), member 6 |
| 别名 | SLC6A6, HTRDC, Sodium- and chloride-dependent taurine transporter, TAUT |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC137128 |
| 基因名称 | SLC6A6/TAUT |
| Gene ID (NCBI) | 6533 |
| RRID | AB_3085896 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31641 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC6A6, also known as TAUT, belongs to the solute carrier 6 (SLC6) family. SLC6A6 is expressed in retinal capillary endothelial cells and mediates the transport of taurine. SLC6A6 can also mediate the transport of beta-alanine, hypotaurine, and gamma-aminobutyric acid. SLC6A6 has 2 isoforms with the molecular mass of 23 and 70 kDa. Sometimes bands of 50 kDa (non-glycosylated) and 75 kDa (glycosylated) may be detected refer to literature (PMID: 35050188).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC6A6/TAUT antibody 26725-1-AP | Download protocol |
| WB protocol for SLC6A6/TAUT antibody 26725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci SLC6A6-Mediated Taurine Uptake Sustains Corneal Epithelial Stem/Progenitor Cell Function to Counteract Age-Related Dysfunction | ||
Placenta Gestational TCBPA exposure impairs placental function and fetal Development: Mechanisms of syncytialization disruption and fetal growth restriction |
