验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, HeLa cells, rat kidney |
| Positive IHC detected in | human kidney tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
29372-1-AP targets SLC6A19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 |
CatNo: Ag31076 Product name: Recombinant human SLC6A19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 142-192 aa of BC148801 Sequence: NSFQEPLPWSDCPLNENQTGYVDECARSSPVDYFWYRETLNISTSISDSGS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 6 (neutral amino acid transporter), member 19 |
| 别名 | B0AT1, HND, Sodium-dependent neutral amino acid transporter B(0)AT1 |
| 计算分子量 | 634 aa, 71 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC148801 |
| 基因名称 | SLC6A19 |
| Gene ID (NCBI) | 340024 |
| RRID | AB_2918286 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q695T7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC6A19, also named as B0AT1, encodes system B(0) transmembrane protein that mediates epithelial resorption of neutral amino acids across the apical membrane in kidney and intestine (PubMed: 15044460). The protein contains 634 amino acids and has 12 predicted transmembrane regions with highly expression in human kidney and small intestine and minimal in pancreas (PubMed: 15286787). The expression of SLC6A19 in intestinal cells depends on angiotensin converting enzyme 2 which is the cellular receptor for SARS-CoV-2 (PubMed: 32132184). Mutations in SLC6A19 cause Hartnup disease (PMID: 15286787, PMID: 15286788).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC6A19 antibody 29372-1-AP | Download protocol |
| WB protocol for SLC6A19 antibody 29372-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Pathol Role of Kir5.1 (Kcnj16) Channels in Regulating Renal Ammonia Metabolism during Metabolic Acidosis in Dahl Salt-Sensitive Rats | ||
J Ethnopharmacol Metabolomics and molecular docking-directed anti-obesity study of the ethanol extract from Gynostemma pentaphyllum (Thunb.) Makino |
