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SLC5A8 Polyclonal antibody

SLC5A8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse , rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29827-1-AP

Print datasheet

Synonyms

AIT, Apical iodide transporter, SLC5A8, SMCT, SMCT1

验证数据展示

  • WB analysis using 29827-1-AP

    WB analysis using 29827-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29827-1-AP (SLC5A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 29827-1-AP

    IHC staining of rat brain using 29827-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29827-1-AP targets SLC5A8 in WB, IHC, ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse , rat
Immunogen SLC5A8 fusion protein Ag30183 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 5 (iodide transporter), member 8
Synonyms AIT, Apical iodide transporter, SLC5A8, SMCT, SMCT1
Calculated Molecular Weight 610 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC110492
Gene Symbol SLC5A8
Gene ID (NCBI) 160728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The sodium-coupled monocarboxylate transporter 1 (SMCT1; SLC5A8), belonging to the SLC5A family, is a 69-kDa protein functions as a Na+-coupled electrogenic transporter for SCFAs. SLC5A mRNA and protein are expressed in the healthy human, rat, and mouse colon. And the protein is localized to apical membrane of the colonocytes. Immunohistochemical studies revealed the selective localization of SMCT1 on the brush border in the villi of the terminal ileum and crypts of the colon in mice.

实验方案

Product Specific Protocols
WB protocol for SLC5A8 antibody 29827-1-APDownload protocol
IHC protocol for SLC5A8 antibody 29827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols