验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, mouse brain tissue, mouse small intestine tissue, COLO 320 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
26407-1-AP targets SLC5A6 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SLC5A6 fusion protein Ag23907 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 5 (sodium-dependent vitamin transporter), member 6 |
| 别名 | Na(+)-dependent multivitamin transporter, SMVT, Sodium-dependent multivitamin transporter |
| 观测分子量 | 69 kDa |
| GenBank蛋白编号 | BC012806 |
| 基因名称 | SLC5A6 |
| Gene ID (NCBI) | 8884 |
| RRID | AB_2880502 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y289 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC5A6, also known as the sodium-dependent multivitamin transporter (hSMVT), is a transmembrane protein that plays a crucial role in the uptake of essential vitamins, including biotin, pantothenic acid, and lipoic acid. This protein is widely expressed in various human tissues, including the placenta, intestine, brain, liver, lung, kidney, cornea, retina, and heart. It uses energy from the transmembrane sodium ion gradient and membrane potential to actively transport these vitamins into cells. Due to different glycosylation modifications, SLC5A6 may exhibit two bands at ~55 kDa and ~69 kDa. (PMID: 20980265, PMID: 25971966)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC5A6 antibody 26407-1-AP | Download protocol |
| IP protocol for SLC5A6 antibody 26407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Metab Vitamin B5 supports MYC oncogenic metabolism and tumor progression in breast cancer | ||
Mol Cell Proteomics Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression. |
