验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, mouse small intestine tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
21628-1-AP targets SLC5A3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag16218 Product name: Recombinant human SLC5A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 533-695 aa of BC146536 Sequence: TPPPTKEQIRTTTFWSKKNLVVKENCSPKEEPYQMQEKSILRCSENNETINHIIPNGKSEDSIKGLQPEDVNLLVTCREEGNPVASLGHSEAETPVDAYSNGQAALMGEKERKKETDDGGRYWKFIDWFCGFKSKSLSKRSLRDLMEEEAVCLQMLEETRQVK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 5 (sodium/myo-inositol cotransporter), member 3 |
| 别名 | SLC5A3, SMIT, SMIT2 |
| 计算分子量 | 718 aa, 80 kDa |
| 观测分子量 | 77 kDa |
| GenBank蛋白编号 | BC146536 |
| 基因名称 | SLC5A3 |
| Gene ID (NCBI) | 6526 |
| RRID | AB_10863123 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P53794 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC5A3 antibody 21628-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma | ||
Nat Cancer Targeting LIPA independent of its lipase activity is a therapeutic strategy in solid tumors via induction of endoplasmic reticulum stress |
