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验证数据展示

  • WB analysis using 28648-1-AP

    WB analysis using 28648-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28648-1-AP (SLC5A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 28648-1-AP

    IHC staining of human kidney using 28648-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28648-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 28648-1-AP

    IHC staining of human kidney using 28648-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28648-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 28648-1-AP

    IF Staining of mouse small intestine using 28648-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SLC5A11 antibody (28648-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 28648-1-AP

    IF Staining of HepG2 using 28648-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC5A11 antibody (28648-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SLC5A11 Polyclonal antibody

SLC5A11 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 28648-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

KST1, Na(+)/myo-inositol cotransporter 2, RKST1, SGLT6, SLGTX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28648-1-AP targets SLC5A11 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC5A11 fusion protein Ag29916 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 5 (sodium/glucose cotransporter), member 11
别名 KST1, Na(+)/myo-inositol cotransporter 2, RKST1, SGLT6, SLGTX
计算分子量 611 aa, 67 kDa
观测分子量70-74 kDa
GenBank蛋白编号BC057780
基因名称 SLC5A11
Gene ID (NCBI) 115584
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWX8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC5A11 (Sodium/myo-inositol cotransporter 2 ), also known as KST1, SMIT2, is a sodium/solute cotransporter-like neuronal protein. In Drosophila, SLC5A11 mediates hunger by regulating potassium channel activity. SLC5A11 has been linked to infantile convulsions and paroxysmal dyskinesia (ICCA syndrome) and benign familial infantile convulsions (BFIC) (PMID: 27397890).

实验方案

Product Specific Protocols
WB protocol for SLC5A11 antibody 28648-1-APDownload protocol
IHC protocol for SLC5A11 antibody 28648-1-APDownload protocol
IF protocol for SLC5A11 antibody 28648-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols