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验证数据展示

  • WB analysis using 14089-1-AP

    WB analysis using 14089-1-AP

    C2C12 cells were subjected to SDS PAGE followed by western blot with 14089-1-AP (SLC5A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14089-1-AP

    IHC staining of human colon cancer using 14089-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC5A11 Polyclonal antibody

SLC5A11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14089-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

KST1, Na(+)/myo-inositol cotransporter 2, RKST1, SGLT6, SLGTX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC2C12 cells
Positive IHC detected inhuman colon cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14089-1-AP targets SLC5A11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 SLC5A11 fusion protein Ag5220 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 5 (sodium/glucose cotransporter), member 11
别名 KST1, Na(+)/myo-inositol cotransporter 2, RKST1, SGLT6, SLGTX
计算分子量 611 aa, 67 kDa
观测分子量 74 kDa
GenBank蛋白编号BC057780
基因名称 SLC5A11
Gene ID (NCBI) 115584
RRIDAB_2189540
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWX8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC5A11 (Sodium/myo-inositol cotransporter 2 ), also known as KST1, SMIT2, is a sodium/solute cotransporter-like neuronal protein. In Drosophila, SLC5A11 mediates hunger by regulating potassium channel activity. SLC5A11 has been linked to infantile convulsions and paroxysmal dyskinesia (ICCA syndrome) and benign familial infantile convulsions (BFIC) (PMID: 27397890).

实验方案

Product Specific Protocols
WB protocol for SLC5A11 antibody 14089-1-APDownload protocol
IHC protocol for SLC5A11 antibody 14089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Clin Endocrinol Metab

Placental Inositol Reduced in Gestational Diabetes as Glucose Alters Inositol Transporters and IMPA1 Enzyme Expression.

Authors - Reshma A Pillai
View Article
humanWB

Front Pharmacol

Quercetin Attenuates Atherosclerosis via Modulating Oxidized LDL-Induced Endothelial Cellular Senescence.

Authors - Yue-Hua Jiang
View Article