验证数据展示
经过测试的应用
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30396-1-AP targets SLC52A2 in IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag33252 Product name: Recombinant human GPR172A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 217-276 aa of BC002917 Sequence: LLPPPPSVPTGELGSGLQVGAPGAEEEVEESSPLQEPPSQAAGTTPGPDPKAYQLLSARS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Solute carrier family 52, riboflavin transporter, member 2 |
| 别名 | GPCR, GPCR41, GPR172A, PAR1, RFT3 |
| 计算分子量 | 46 kDa |
| GenBank蛋白编号 | BC002917 |
| 基因名称 | SLC52A2 |
| Gene ID (NCBI) | 79581 |
| RRID | AB_3742338 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HAB3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC52A2 (Also known as RFVT2, RFT3) is a membrane protein that belongs to the riboflavin transporter family. SLC52A2 is a transmembrane protein that mediates cellular uptake of riboflavin. Humans are unable to synthesize vitamin B2/riboflavin and must obtain it via intestinal absorption (PubMed:20463145). Mutations in SLC52A2 have been associated with Brown-Vialetto-Van Laere syndrome 2 (BVVLS2) - an autosomal recessive progressive neurologic disorder characterized by deafness, bulbar dysfunction, and axial and limb hypotonia (PMID: 22864630, 23243084).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC52A2 antibody 30396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
